Anti-GAPDH Mouse mAb

Antibody type:Loading control antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgM

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Human,Monkey,Rat,Mouse,Saccharomyces cerevisiae,Pichia pastoris,Hamster

Gene Name:gapdh

Synonyms:G3PD;GAPD.

Gene Synonyms:38 kDa BFA-dependent ADP-ribosylation substrate;aging associated gene 9 protein;Aging-associated gene 9 protein;BARS-38;cb609;EC 1.2.1.12;G3P_HUMAN;G3PD;G3PDH;GAPD;GAPDH; GAPDH;Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogen

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human GAPDH protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of GAPDH and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:7E4-H6-H6

Dilution:WB 1:5000

Mole Mass:37kDa

Location:Cytoplasm >> cytosol.Nucleus.Cytoplasm >> perinuclear region.Membrane.Cytoplasm >> cytoskeleton.

Concentration:

Sequence Similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Gene Id:2597

SwissProt ID:P04406

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Ascites containing with 0.03% Proclin300 and 50% glycerol.

Background:Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.