Anti-GFP-Tag Mouse Monoclonal

Antibody type:Tag antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Mouse IgG1

Host:Mouse

Application:WB,IHC,ICC/IF,IP

Purify method:Affinity Chromatography

Species:All

Gene Name:

Synonyms:GFP ; yfp ; Green fluorescent protein ;

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:Full-length GFP protein

Antigen Synonyms:

Clonality:

Source:

Reaction:GFP-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal GFP-tagged proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:3000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:100

Mole Mass:27kDa

Location:

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:Q963I9

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -23°C long term. Avoid freeze / thaw cycle.

Background:Green fluorescent protein(GFP) has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.