Anti-Galectin-3 Mouse mAb (5G5)
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Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:IHC,ELISA
Purify method:Affinity purified
Species:Human
Gene Name:LGALS3
Synonyms:35KD lectin, CBP35,GBP,IgE-binding protein, L31,Laminin binding protein,Lectin L29
Gene Synonyms:
Gene Full Name:
Gene Infomation:
Antigen:Purified recombinant protein expressed in E.coli.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:
Reaction:The Galetcin-3 antibody can recognizeendogenous Human Galetcin-3 proteins.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:5G5-3H1-3C10
Dilution:IHC1:100-200 ELISA 10,000-50,000 (Optimal dilutions should be determined by the end user)
Mole Mass:26kDa
Location:Cytoplasm, Nucleus, Secreted, Spliceosome
Concentration:
Sequence Similarity:
Gene Id:3958
SwissProt ID:P17931
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Background:Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis . In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.