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Anti-HAUSP/USP7 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Human

Gene Name:usp7

Synonyms:TEF1;HAUSP.

Gene Synonyms:Deubiquitinating enzyme 7;HAUSP;herpes virus associated;Herpes virus associated ubiquitin specific protease;Herpesvirus-associated ubiquitin-specific protease;TEF 1;tef-1;TEF1;Ubiquitin carboxyl terminal hydrolase 7;Ubiquitin carboxyl-terminal hydrolase 7

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human HAUSP / USP7 protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of HAUSP / USP7 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

  • Western blot (1:100 to 1:500)
  • Immunofluorescence (1:50 to 1:400)
  • Immunohistochemistry (1:200 to 1:500)
  • Flow cytometry analysis (1:200 to 1:500)
  • Enzyme-linked Immunosorbent Assay (1:100-1:5000)
NoteUsers are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:3E4-D10-E8

Dilution:WB 1:200

Mole Mass:135kDa

Location:Nucleus.Cytoplasm.Nucleus ? PML body.Note:Present in a minority of ND10 nuclear bodies.Association with ICP0/VMW110 at early times of infection leads to an increased proportion of USP7-containing ND10.Colocalizes with ATXN1 in the nucleus.Colocalized with

Concentration:

Sequence Similarity:Belongs to the peptidase C19 family.Contains 1 MATH domain.

Gene Id:7874

SwissProt ID:Q93009

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Ascites containing with 0.03% Proclin300 and 50% glycerol.

Background:Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN and DAXX (PubMed:11923872, PubMed:15053880, PubMed:16964248, PubMed:18716620, PubMed:25283148).

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.