Anti-HDAC2 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB,IF

Purify method:Affinity purified

Species:Human,Mouse,Rat,Monkey

Gene Name:HDAC2

Synonyms:HD2; RPD3; YAF1;HDAC2.

Gene Synonyms:D10Wsu179e ;HD 2 ;HD2 ;HDAC 2 ;Hdac2 ;HDAC2_HUMAN ;Histone deacetylase 2 (HD2) ;Histone deacetylase 2 ;OTTHUMP00000017046 ;OTTHUMP00000227077 ;OTTHUMP00000227078 ;RPD3 ;transcriptional regulator homolog RPD3 ;YAF1 ;YY1 associated factor 1 ;YY1 transcription factor binding protein ;Yy1bp.

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human HDAC2 protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:This antibody detects endogenous levels of HDAC2 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:2D9-F6-G7

Dilution:WB 1:1000

Mole Mass:60kDa

Location:Nucleus.

Concentration:1.0mg/ml

Sequence Similarity:Belongs to the histone deacetylase family. HD type 1 subfamily.

Gene Id:3066

SwissProt ID:Q92769

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.