Anti-HDAC6 Rabbit pAb
- 100ug (NP21136) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,IHC,ICC/IF,IP
Purify method:Affinity Chromatography
Species:Human
Gene Name:
Synonyms:HD 6; HDAC 6; Histone deacetylase 6 (HD6); JM 21;
Gene Synonyms:
Gene Full Name:
Gene Infomation:Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.
Antigen:A synthesized peptide derived from human HDAC6
Antigen Synonyms:
Clonality:Polyclonal antibody
Source:
Reaction:HDAC6 Antibody detects endogenous levels of HDAC6
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB 1:5000~1:20000 IHC 1:50~1:200 ICC/IF 1:100~1:500 IP 1:50
Mole Mass:160kDa
Location:Nucleus;
Concentration:
Sequence Similarity:Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
Gene Id:
SwissProt ID:Q9UBN7
Unigene:
Nucleotide Accession:
Tissue specificity:HDAC6 Antibody detects endogenous levels of HDAC6
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Background:Involved in the regulation of many cellular processes, including cell migration, immune synapse formation, viral infection, and degradation of misfolded proteins. HDAC6 binds to both poly-ubiquitinated misfolded proteins and dynein motors, facilitating the transport of misfolded proteins to the aggresome. Required for subsequent recruitment of the autophagic machinery and clearance of aggresomes from the cell. Plays a key role in the protection against the deleterious effects of pathological protein aggregation that occurs in various diseases.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.