Anti-HIF-1 betaRabbit pAb
- 100ug (NP21714) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,IHC
Purify method:Affinity Chromatography
Species:Human
Gene Name:
Synonyms:ARNT;HIF-1beta;HIF1B;HIF1BETA;TANGO;bHLHe2;
Gene Synonyms:
Gene Full Name:
Gene Infomation:ABCG1 (also designated ABC8 or human white gene), a member of the evolutionary conserved family of ATP-binding cassette (ABC) transporters, exhibits high homology with the Drosophila white gene. ABC transporters couple the energy of ATP hydrolysis to the translocation of various molecules across biological membranes. These proteins contain characteristic ATP-binding domains and transmembrane domains which form a channel-like structure for transport. ABCG1 functions to regulate cholesterol and phospholipid transport in macrophages. ABCG1 is highly expressed in several tissues, including brain, spleen, lung and placenta, and has been localized to the cell surface and intracellular compartments of cholesterol-laden macrophages.
Antigen:A synthesized peptide derived from human HIF-1 beta
Antigen Synonyms:
Clonality:Polyclonal antibody
Source:
Reaction:HIF-1 beta Antibody detects endogenous levels of total HIF-1 beta
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB 1:500~1:2000 IHC 1:500~1:2000
Mole Mass:87kDa
Location:Golgi apparatus membrane > Multi-pass membrane protein;
Concentration:
Sequence Similarity:Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Gene Id:
SwissProt ID:P27540
Unigene:
Nucleotide Accession:
Tissue specificity:HIF-1 beta Antibody detects endogenous levels of total HIF-1 beta
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Background:HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex. Together with AhR, HIF-1β plays an important role in xenobiotics metabolism. In addition, a chromosomal translocation leading to a TEL-ARNT fusion protein is associated with acute myeloblastic leukemia. Studies also found that ARNT/HIF-1β expression levels decrease significantly in pancreatic islets from patients with type 2 diabetes, suggesting that HIF-1β plays an important role in pancreatic β-cell function.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.