Anti-HIF-2 alpha Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,ICC/IF,FC

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:bHLHe73;ECYT4;EPAS 1;EPAS1;HIF2A;HLF;MOP 2;PASD2

Gene Synonyms:

Gene Full Name:

Gene Infomation:Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5-[AG]CGTG-3 within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

Antigen:A synthesized peptide derived from human HIF-2 alpha

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:HIF-2-alpha Antibody detects endogenous levels of total HIF-2-alpha

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:2000 ICC/IF 1:50~1:200 FC 1:50

Mole Mass:96kDa

Location:Nucleus speckle;

Concentration:

Sequence Similarity:Contains 2 PAS (PER-ARNT-SIM) domains.

Gene Id:

SwissProt ID:Q99814

Unigene:

Nucleotide Accession:

Tissue specificity:HIF-2-alpha Antibody detects endogenous levels of total HIF-2-alpha

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.