Anti-HNMT Rabbit pAb
- 100ug (NP15026) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,IHC,IP
Purify method:Affinity purified
Species:Human,Mouse
Gene Name:HNMT
Synonyms:HMT; MRT51; HNMT-S1; HNMT-S2
Gene Synonyms:
Gene Full Name:histamine N-methyltransferase
Gene Infomation:In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008]
Antigen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human HNMT (NP_008826.1).
Antigen Synonyms:Histamine N-methyltransferase
Clonality:Polyclonal antibody
Source:Human
Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB1:500 -1:2000; IHC1:50 -1:200; IP1:20 -1:50(Optimal dilutions should be determined by the end user)
Mole Mass:27kDa
Location:Cytoplasm
Concentration:
Sequence Similarity:
Gene Id:
SwissProt ID:P50135
Unigene:3176
Nucleotide Accession:
Tissue specificity:
Storage:Store at -20°C. Avoid freeze / thaw cycles.
Buffer condition:PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background:
Molar Function:Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.