Anti-HP-1γ Mouse mAb (6D8)
- 100ug (NP14102) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:WB,IHC
Purify method:Affinity purified
Species:Human,Rat,Mouse
Gene Name:CBX3
Synonyms:CBX3, Chromobox homolog 3, HECH, HP1 gamma
Gene Synonyms:
Gene Full Name:
Gene Infomation:
Antigen:Purified recombinant protein expressed in E.coli.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:
Reaction:The HP-1gamma-mouse monoclonal Antibody can detects endogenous HP-1gamma-proteins.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:6D8-10D10-5A9
Dilution:WB 1:1,000-2,000 IHC 1:100-200 (Optimal dilutions should be determined by the end user)
Mole Mass:24kDa
Location:Nucleus
Concentration:
Sequence Similarity:
Gene Id:11335
SwissProt ID:Q13185
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Background:Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitement of NIPBL to sites of DNA damage at double-strand breaks (DSBs) (PubMed:28167679).
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.