Anti-HPRT Rabbit pAb
- 100ug (NP20498) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,IHC,IP
Purify method:Affinity Chromatography
Species:Human,Mouse,Rat
Gene Name:
Synonyms:HGPRT; HGPRTase; HPRT1;
Gene Synonyms:
Gene Full Name:
Gene Infomation:Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purin
Antigen:A synthesized peptide derived from human HPRT
Antigen Synonyms:
Clonality:Polyclonal antibody
Source:
Reaction:HPRT Antibody detects endogenous levels of total HPRT
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB 1:1000~1:5000 IHC 1:50~1:200 IP 1:50
Mole Mass:25kDa
Location:Cytoplasm;
Concentration:
Sequence Similarity:Belongs to the purine/pyrimidine phosphoribosyltransferase family.
Gene Id:
SwissProt ID:P00492
Unigene:
Nucleotide Accession:
Tissue specificity:HPRT Antibody detects endogenous levels of total HPRT
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Background:Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.