Anti-HSP27 (Ab-82) Rabbit pAb

100ug (NP13602) Please inquiry

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Rabbit

Application:WB,IHC

Purify method:Unpurification

Species:Human

Gene Name:hsp27

Synonyms:28 kDa heat shock protein Estrogen-regulated 24 kDa protein Growth-related 25 kDa protein HSP 27 HSP25

Gene Synonyms:

Gene Full Name:

Gene Infomation:Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Antigen:Peptide sequence around aa. 80~84 (Q-L-S-S-G) derived from Human HSP27.

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

Western blot (1:100 to 1:500)
Immunofluorescence (1:50 to 1:400)
Immunohistochemistry (1:200 to 1:500)
Flow cytometry analysis (1:200 to 1:500)
Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB::1:500-1:1000IHC::1:50-1:100

Mole Mass:27

Location:

Concentration:1.0mg/mL

Sequence Similarity:

Gene Id:3315

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:The antibody detects endogenous level of total HSP27 protein.

Storage:

Buffer condition:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:

Molar Function:

Western Blot:

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.