Anti-HSPB8/HSP22 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:CMT2L; CRYAC; DHMN2; E2IG1; H11; HMN2; HSPB8; HSP22;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Proteins in the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex are integral membrane proteins involved in vesicle transport and membrane fusion by pairing of vesicular SNAREs (v-SNAREs) with cognate target SNAREs (t-SNAREs). Vesicle associated membrane protein 8 (VAMP8), also known as endobrevin, is a v-SNARE originally found preferentially localized to early endosomes. VAMP8 knockout mice did not show abnormal endosomal vesicular trafficking, perhaps having a redundant role with other VAMP family members. Instead, research studies have shown that VAMP8 is widely expressed in exocrine tissues and has a critical role in the exocytosis pathways of a variety of cells.

Antigen:A synthesized peptide derived from human HSPB8/HSP22

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:HSPB8/HSP22 Antibody detects endogenous levels of total HSPB8/HSP22

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:2000 IHC 1:50~1:100 ICC/IF 1:50~1:100

Mole Mass:22kDa

Location:Cell membrane > Single-pass type IV membrane protein;

Concentration:

Sequence Similarity:Belongs to the synaptobrevin family.

Gene Id:

SwissProt ID:Q9UJY1

Unigene:

Nucleotide Accession:

Tissue specificity:HSPB8/HSP22 Antibody detects endogenous levels of total HSPB8/HSP22

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:HSPB8 (HSP22) is a member of the small heat shock protein superfamily and the human protein is most closely related to HSP27. Similar to most other small HSPs (sHSPs), HSPB8 is predominantly transcribed in skeletal muscle and heart. In a two hybrid screen, HSPB8 interacted preferentially with a triple aspartate form of HSP27 which mimics HSP27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type HSP27.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.