Anti-Hexokinase II Mouse mAb
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Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:WB
Purify method:Affinity purified
Species:Human
Gene Name:hk2
Synonyms:HKII; HXK2
Gene Synonyms:DKFZp686M1669 ;Hexokinase 2 ;Hexokinase 2 muscle ;Hexokinase type II ;Hexokinase-2 ;HK 2 ;HK II ;HK2 ;HKII ;HxK 2 ;HxK2 ;HXK2_HUMAN ;Muscle form hexokinase .
Gene Full Name:
Gene Infomation:
Antigen:Purified recombinant human Hexokinase II protein fragments expressed in E.coli.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:Human
Reaction:This antibody detects endogenous levels of Hexokinase II and does not cross-react with related proteins.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:3B9-F8-F10
Dilution:WB 1:1000
Mole Mass:102kDa
Location:Mitochondrion outer membrane.
Concentration:
Sequence Similarity:Belongs to the hexokinase family. Contains 2 hexokinase type-1 domains. Contains 2 hexokinase type-2 domains.
Gene Id:3099
SwissProt ID:P52789
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Background:Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.