Anti-His-Tag Mouse Monoclonal
- 100ug (NP14428) Please inquiry
Antibody type:Tag antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Mouse IgG1
Host:Mouse
Application:WB,ICC/IF,IP
Purify method:Affinity Chromatography
Species:All
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Gene Infomation:
Antigen:A synthesized peptide
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Clonality:
Source:
Reaction:His-Tag Mouse Monoclonal antibody detects endogenous levels of internal, C-terminal, or N-terminal 6*His -tagged proteins.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB 1:1000~1:5000 ICC/IF 1:100~1:200 IP 1:100
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Sequence Similarity:
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Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Background:His-Tag antibody is part of the Tag series of antibodies, the best quality in the research.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.