Anti-His-tag Mouse mAb

Antibody type:Tag antibody

Label:Unconjugated

Modification:

Isotype:IgG1

Host:Mouse

Application:WB

Purify method:Unpurification

Species:

Gene Name:his

Synonyms:6 His epitope tag;Hexa His tag;HHHHHH epitope tag;HHHHHH tag;His tag;Polyhistidine Tag.

Gene Synonyms:

Gene Full Name:

Gene Infomation:The 6xHis (H-H-H-H-H-H) motif is used as a epitope tag on many recombinant proteins for efficient coupling to Ni2affinity resins and purification by one step chromatography. The antibody recognizes the 6xHis-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Cellular localization: Depends upon the localization of the parent protein tagged with hexahistidine.

Antigen:Synthetic peptide corresponding to fragment (HHHHHH) His Tag

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Artificial tag

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:1H3-A2

Dilution:WB：1/500~1/2000

Mole Mass:

Location:

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:This antibody specifically recognizes the 6X His-Tag at C-terminal or N-terminal of recombinant proteins,which can be used to detect His-Tag fusion protein of various expression vector.

Storage:

Buffer condition:

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.