Anti-Histone H2A.X(Phospho-Ser139) Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Phosphoralated

Isotype:IgG2a

Host:Mouse

Application:WB,IF

Purify method:Affinity purified

Species:Human,Mouse

Gene Name:h2ax

Synonyms:H2AX; H2A.X; H2A/X

Gene Synonyms:H2A histone family, member X;H2A.X;H2a/x;H2AFX;H2AX;H2AX histone;H2AX_HUMAN;Histone H2A.X;Histone H2AX

Gene Full Name:

Gene Infomation:

Antigen:Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of H2A.X only when phosphorylated at serine 139.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:2A9-B5

Dilution:WB 1:2000,ICC 1:400,Other 1:400

Mole Mass:15kDa

Location:Nucleus. Chromosome

Concentration:4.2mg/ml

Sequence Similarity:Belongs to the histone H2A family.

Gene Id:3014

SwissProt ID:P16104

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.