Anti-Histone H2A.X(Phospho-Ser139) Mouse mAb
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Antibody type:Primary antibody
Label:Unconjugated
Modification:Phosphoralated
Isotype:IgG1
Host:Mouse
Application:WB,IF
Purify method:Affinity purified
Species:Mouse
Gene Name:h2ax
Synonyms:H2AX; H2A.X; H2A/X
Gene Synonyms:H2A histone family, member X;H2A.X;H2a/x;H2AFX;H2AX;H2AX histone;H2AX_HUMAN;Histone H2A.X;Histone H2AX
Gene Full Name:
Gene Infomation:
Antigen:Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:Human
Reaction:This antibody detects endogenous levels of H2A.X only when phosphorylated at serine 139.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:3E11-1H6-G3
Dilution:WB 1:2000,ICC 1:400
Mole Mass:15kDa
Location:Nucleus. Chromosome
Concentration:4.73mg/ml
Sequence Similarity:Belongs to the histone H2A family.
Gene Id:3014
SwissProt ID:P16104
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Background:Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.