Anti-Histone H2A.X(Phospho-Ser139) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Rabbit

Application:WB,ICC,IHC

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:h2afx

Synonyms:AW228881;H2A histone family member X;H2A.FX;H2A.X;H2A/X;H2AFX;H2AX;H2AX histone;H2AX_HUMAN;Hist5.2ax;Histone 2A;Histone 2AX;Histone H2A.x;RGD1566119.

Gene Synonyms:H2AX; H2A.X; H2A/X

Gene Full Name:

Gene Infomation:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008]

Antigen:The antiserum was produced against synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139(Q-A-SP-Q-E).

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:Human

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:

Mole Mass:16

Location:Nucleus. Chromosome

Concentration:

Sequence Similarity:Belongs to the histone H2A family.

Gene Id:3014

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:This antibody detects endogenous levels of H2A.X and does not cross-react with related proteins.

Storage:

Buffer condition:10mM PBS with 0.02% sodium azide, 50% glycerol.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.