Anti-Histone H3.3Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:H3.3; H3.3A; H33_HUMAN ; H3F3; Histone H3.3 ; H3 histone family 3A; H3 histone family 3B

Gene Synonyms:

Gene Full Name:

Gene Infomation:PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Antigen:A synthesized peptide derived from human Histone H3.3

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Histone H3.3 Antibody detects endogenous levels of total Histone H3.3

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:1000 IHC 1:500~1:1000 ICC/IF 1:500~1:1000

Mole Mass:15kDa

Location:Melanosome;

Concentration:

Sequence Similarity:Contains 1 PPIase cyclophilin-type domain.

Gene Id:

SwissProt ID:P84243

Unigene:

Nucleotide Accession:

Tissue specificity:Histone H3.3 Antibody detects endogenous levels of total Histone H3.3

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.