Anti-Histone H3 Rabbit pAb
- 100ug (NP21146) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,IHC,ICC/IF,ChIP
Purify method:Affinity Chromatography
Species:Human,Mouse,Rat
Gene Name:
Synonyms:Histone H3.1, Histone H3, HIST1H3A;
Gene Synonyms:
Gene Full Name:
Gene Infomation:Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase.
Antigen:A synthesized peptide derived from human Histone H3
Antigen Synonyms:
Clonality:Polyclonal antibody
Source:
Reaction:Histone H3 Antibody detects endogenous levels of Histone H3
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB 1:1000~1:2000 IHC 1:1000~1:2000 ICC/IF 1:1000~1:2000 IP 1:50 ChIP
Mole Mass:17kDa
Location:Secreted;
Concentration:
Sequence Similarity:Belongs to the intercrine alpha (chemokine CxC) family.
Gene Id:
SwissProt ID:P68431
Unigene:
Nucleotide Accession:
Tissue specificity:Histone H3 Antibody detects endogenous levels of Histone H3
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Background:Belongs to the histone H3 family. Play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.