Anti-Histone H4 (acetyl K5) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF,IP

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:H4F2; HIST; HIST1H4A; Histone H4.AC-H4K5;H4K5;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Regulates MYC expression by binding to a single-stranded far-upstream element upstream of the MYC promoter. May act both as activator and repressor of transcription.

Antigen:A synthesized peptide derived from human Histone H4 (acetyl K5)

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Histone H4 (acetyl K5) Antibody detects endogenous levels of total Histone H4 (acetyl K5)

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

Mole Mass:11kDa

Location:Nucleus;

Concentration:

Sequence Similarity:Contains 4 KH domains.

Gene Id:

SwissProt ID:P62805

Unigene:

Nucleotide Accession:

Tissue specificity:Histone H4 (acetyl K5) Antibody detects endogenous levels of total Histone H4 (acetyl K5)

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.