Anti-Hsp20 Rabbit pAb
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Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,IHC,FC
Purify method:Affinity Chromatography
Species:Human,Mouse,Rat
Gene Name:
Synonyms:HSPB6; HEL55; Hsp20; Heat-shock 20 kDa like-protein p20;
Gene Synonyms:
Gene Full Name:
Gene Infomation:Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
Antigen:A synthesized peptide derived from human Hsp20
Antigen Synonyms:
Clonality:Polyclonal antibody
Source:
Reaction:Hsp20 Antibody detects endogenous levels of total Hsp20
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB 1:5000~1:10000 IHC 1:50~1:200 FC 1:50
Mole Mass:17kDa
Location:Cell membrane;
Concentration:
Sequence Similarity:Belongs to the adenomatous polyposis coli (APC) family.
Gene Id:
SwissProt ID:O14558
Unigene:
Nucleotide Accession:
Tissue specificity:Hsp20 Antibody detects endogenous levels of total Hsp20
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Background:HSP20 a small heat shock protein of the HSP20 family. Note: This description may include information from UniProtKB.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.