Anti-Hsp27 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB,ICC

Purify method:Affinity purified

Species:Human,Monkey

Gene Name:hspb1

Synonyms:CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067

Gene Synonyms:Heat shock 27kDa protein;28 kDa heat shock protein;CMT2F;DKFZp586P1322;Estrogen regulated 24 kDa protein;Estrogen-regulated 24 kDa protein;Heat shock 25kDa protein 1;Heat shock 27 kDa protein;Heat shock 27kD protein 1;Heat shock 27kDa protein 1;Heat shock

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human Hsp27 protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of Hsp27 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:7E5-1F9-D9

Dilution:WB 1:1000,ICC 1:300

Mole Mass:27kDa

Location:Cytoplasm. Nucleus. Cytoplasm › cytoskeleton › spindle.

Concentration:

Sequence Similarity:Belongs to the small heat shock protein (HSP20) family.

Gene Id:3315

SwissProt ID:P04792

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide 50% glycerol 0.1%BSA

Background:Involved in stress resistance and actin organization.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.