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Anti-Hsp60 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB,IF,IP

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:hsp60

Synonyms:HLD4;CPN60;GROEL;HSP60;HSP65;SPG13;HSP-60;HuCHA60.

Gene Synonyms:60 kDa chaperonin;60 kDa heat shock protein mitochondrial;60 kDa heat shock protein, mitochondrial;CH60_HUMAN;Chaperonin 60;Chaperonin;Chaperonin, 60-KD;CPN 60;CPN60;fa04a05;GROEL;GroEL Homolog;Heat shock 60kD protein 1 chaperonin;Heat shock 60kDa protein

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human Hsp60 protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of Hsp60 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

  • Western blot (1:100 to 1:500)
  • Immunofluorescence (1:50 to 1:400)
  • Immunohistochemistry (1:200 to 1:500)
  • Flow cytometry analysis (1:200 to 1:500)
  • Enzyme-linked Immunosorbent Assay (1:100-1:5000)
NoteUsers are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:6C8-G1-B10

Dilution:WB 1:1000,ICC 1:100,IP 1:100

Mole Mass:60kDa

Location:Mitochondrion matrix.

Concentration:2.6mg/ml

Sequence Similarity:Belongs to the chaperonin (HSP60) family.

Gene Id:3329

SwissProt ID:P10809

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:Implicated in mitochondrial protein import and macromolecular assembly.May facilitate the correct folding of imported proteins.May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.