Anti-Human EGF Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:ELISA

Purify method:Affinity purified

Species:Human

Gene Name:egf

Synonyms:URG; HOMG4.

Gene Synonyms:AI790464 ;Beta urogastrone ;Epidermal Growth Factor ;HOMG4 ;OTTHUMP00000219721 ;OTTHUMP00000219722 ;Pro epidermal growth factor ;Pro epidermal growth factor precursor (EGF) ;URG ;Urogastrone .

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant Human EGF protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects Human EGF.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:5E9-A10-A6

Dilution:

Mole Mass:

Location:Membrane; Single-pass type I membrane protein.

Concentration:2.0mg/mL

Sequence Similarity:Contains 9 EGF-like domains.PROSITE-ProRule annotation ;Contains 9 LDL-receptor class B repeats.PROSITE-ProRule annotation .

Gene Id:1950

SwissProt ID:P01133

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.03% Proclin300 and 20% glycerol.

Background:EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.