Anti-IKBKB Mouse mAb
- 100ug (NP10432) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Mouse IgG1
Host:Mouse
Application:WB,IHC
Purify method:Ascitic fluid
Species:Human
Gene Name:IKBKB
Synonyms:IKBKB
Gene Synonyms:
Gene Full Name:
Gene Infomation:
Antigen:Purified recombinant fragment of IKBKB expressed in E. Coli.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:
Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:10A2C5B3
Dilution:WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: N/A; FCM: N/A; Elisa: 1/10000
Mole Mass:
Location:
Concentration:
Sequence Similarity:
Gene Id:3551
SwissProt ID:O14920
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Ascitic fluid containing 0.03% sodium azide.
Background:IKBKB(Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta, also called IKK2/IKKB), is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK-beta has been shown to activate NF-kB and phosphorylate IKB-alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK-beta is essential for activation of IKK by TNF and IL1. Once activated, IKK-beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK-beta activity can also be regulated by MEKK-1.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.