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Anti-IL-8 Mouse mAb (4B6)

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:IHC

Purify method:Affinity purified

Species:Human,Rat,Mouse

Gene Name:CXCL8

Synonyms:9E3,CXCL8,CEF-4,GCP-1,Granulocyte chemotactic protein 1

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:Synthetic peptide conjugated to KLH.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:The IL-8 mouse monoclonal antibody can recognizeendogenous IL-8 proteins.

Form:Liquid

Tested Applications:

  • Western blot (1:100 to 1:500)
  • Immunofluorescence (1:50 to 1:400)
  • Immunohistochemistry (1:200 to 1:500)
  • Flow cytometry analysis (1:200 to 1:500)
  • Enzyme-linked Immunosorbent Assay (1:100-1:5000)
NoteUsers are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:4B6-9G3-7D9

Dilution:IHC 1:100-200 (Optimal dilutions should bedetermined by the end user)

Mole Mass:11kDa

Location:Secreted

Concentration:

Sequence Similarity:

Gene Id:3576

SwissProt ID:P10145

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.