Anti-KDM1/LSD1 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB,ICC,IP

Purify method:Affinity purified

Species:Human,Monkey

Gene Name:kdm1a

Synonyms:AOF2;KDM1;LSD1;BHC110.

Gene Synonyms:Amine oxidase (flavin containing) domain 2;AOF2;BHC110;BRAF35 HDAC complex protein BHC110;BRAF35-HDAC complex protein BHC110;FAD binding protein BRAF35 HDAC complex, 110 kDa subunit;Flavin-containing amine oxidase domain-containing protein 2;KDM 1;KDM1;Kd

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human KDM1/LSD1 protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of KDM1/LSD1 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:1E5-H2

Dilution:WB 1:1000,ICC 1:100

Mole Mass:110kDa

Location:Nucleus

Concentration:

Sequence Similarity:Belongs to the flavin monoamine oxidase family.Contains 1 SWIRM domain.

Gene Id:23028

SwissProt ID:O60341

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.