Anti-KLHL22 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Mouse IgG1

Host:Mouse

Application:WB,ICC

Purify method:Ascitic fluid

Species:Human

Gene Name:KLHL22

Synonyms:KELCHL; KLHL22

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant fragment of human KLHL22 expressed in E. Coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:7B10

Dilution:WB: 1/500 - 1/2000; IHC: N/A; ICC: 1/200 - 1/1000; FCM: N/A; Elisa: 1/10000

Mole Mass:72kDa

Location:

Concentration:

Sequence Similarity:

Gene Id:84861

SwissProt ID:Q53GT1

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Ascitic fluid containing 0.03% sodium azide.

Background:KLHL22 (kelch-like protein 22) is a 634 amino acid protein that is related to the Drosophilakelch protein, which is required to maintain Actin organization in ovarian ring canals. Mutations affecting Kelch function result in the failure of Kelch to associate with the ring canals and subsequent female sterility. Human KLHL22 protein contains six kelch repeats and one BTB (POZ) domain. The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (Poxvirus and Zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. There are two isoforms of KLHL22 that are produced as a result of alternative splicing events.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.