Anti-Keratin(50-60kDa) Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:krt5

Synonyms:K5; CK5; DDD; DDD1; EBS2; KRT5A

Gene Synonyms:58 kDa cytokeratin; CK 5; CK-5; CK5; Cytokeratin-5; Cytokeratin5; DDD; DDD1; EBS 2; EBS2; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K2C5_HUMAN; K5; Keratin 5 (epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockay

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human KRT5 beta protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of Keratin(50-60kDa) proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:7H5-B5-B6-G8

Dilution:WB 1:2000

Mole Mass:62kDa

Location:Intracellular,Cytoplasm,Cytoskeleton.

Concentration:

Sequence Similarity:Belongs to the intermediate filament family.

Gene Id:3852

SwissProt ID:P13647

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.