Anti-Keratin8/18 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG2a

Host:Mouse

Application:WB,ICC

Purify method:Affinity purified

Species:Human

Gene Name:krt8/18

Synonyms:card2;Cardiac autoantigen 2 120kD;CK 8;CK-8;ck8;Cyk 8;cyk8;CYKER;Cytokeratin 8;Cytokeratin endo A;Cytokeratin-8; Cytokeratin8; DreK8;EndoA; k0;k2c8; K2C8_HUMAN;k8;Keratin 8;Keratin type ii cytoskeletal 8;Keratin, type II cytoskeletal 8;Keratin-8;Keratin8;KO;Krt 2.8;Krt8; krt8;MGC118110;MGC174782;MGC53564;MGC85764;sb:cb186;Type-II keratin Kb8./Cell proliferation inducing gene 46 protein;Cell proliferation inducing protein 46;Cell proliferation-inducing gene 46 protein;CK 18;CK-18;CK18;CYK 18;CYK18;Cytokeratin 18;Cytokeratin endo B;Cytokeratin-18;K 18;K18;K1C18_HUMAN;Keratin 18;Keratin D;keratin, type I cytoskeletal 18;Keratin-18;KRT18.

Gene Synonyms:K8; KO; CK8; CK-8; CYK8; K2C8; CARD2/K18; CYK18

Gene Full Name:

Gene Infomation:This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]/KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Antigen:Recombinant protein corresponding to full length Keratin8 of human.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:

Mole Mass:55/48

Location:Cytoplasm. Nucleus › nucleoplasm. Nucleus matrix./Cytoplasm › perinuclear region. Nucleus › nucleolus.

Concentration:1mg/ml

Sequence Similarity:Belongs to the intermediate filament family.

Gene Id:3856,3875

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:This antibody detects endogenous levels of keratin8/18.

Storage:

Buffer condition:Each vial contains 100 μg IgG in 100 μl of citrate-tris-HCl buffer pH7.0 with 0.02% Proclin 300

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.