Anti-Kif 7 Mouse mAb (2A7)

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:IHC

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:KIF7

Synonyms:kinesin like protein KIF7, EQYK340, kif7

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:Synthetic peptide conjugated to KLH.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:The Kif 7 mouse monoclonal antibody candetectendogenous Kif 7 proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:2A7-10B8-9A9

Dilution:IHC 1:200 (Optimal dilutions should be determined by the end user)

Mole Mass:

Location:Cell projection, Cilium, Cytoplasm, Cytoskeleton

Concentration:

Sequence Similarity:

Gene Id:374654

SwissProt ID:Q2M1P5

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:Essential for hedgehog signaling regulation: acts as both a negative and positive regulator of sonic hedgehog (Shh) and Indian hedgehog (Ihh) pathways, acting downstream of SMO, through both SUFU-dependent and -independent mechanisms (PubMed:21633164). Involved in the regulation of microtubular dynamics. Required for proper organization of the ciliary tip and control of ciliary localization of SUFU-GLI2 complexes . Required for localization of GLI3 to cilia in response to Shh. Negatively regulates Shh signaling by preventing inappropriate activation of the transcriptional activator GLI2 in the absence of ligand. Positively regulates Shh signaling by preventing the processing of the transcription factor GLI3 into its repressor form. In keratinocytes, promotes the dissociation of SUFU-GLI2 complexes, GLI2 nuclear translocation and Shh signaling activation . Involved in the regulation of epidermal differentiation and chondrocyte development .

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.