Anti-Ku70 Mouse mAb
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Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:IgG2b
Host:Mouse
Application:WB,ICC
Purify method:Affinity purified
Species:Human
Gene Name:xrcc6
Synonyms:ML8;KU70;TLAA;CTC75;CTCBF;G22P1.
Gene Synonyms:5''-deoxyribose-5-phosphate lyase Ku70;5''-dRP lyase Ku70;70 kDa subunit of Ku antigen;ATP dependent DNA helicase 2 subunit 1;ATP dependent DNA helicase II 70 kDa subunit;ATP-dependent DNA helicase 2 subunit 1;ATP-dependent DNA helicase II 70 kDa subunit;
Gene Full Name:
Gene Infomation:
Antigen:Purified recombinant human Ku70 protein fragments expressed in E.coli.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:Human
Reaction:This antibody detects endogenous levels of Ku70 and does not cross-react with related proteins.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:6H10-E9-G7
Dilution:WB 1:1000,ICC 1:200
Mole Mass:70kDa
Location:Nucleus.Chromosome.
Concentration:
Sequence Similarity:Belongs to the ku70 family.Contains 1 Ku domain.Contains 1 SAP domain.
Gene Id:2547
SwissProt ID:P12956
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Background:It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.