Anti-LC3A Mouse mAb
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Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:WB
Purify method:Affinity purified
Species:Transfected
Gene Name:lc3a
Synonyms:LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Gene Synonyms:ATG8F; Autophagy related protein LC3 A; Autophagy related protein LC3 B; Autophagy related ubiquitin like modifier LC3 A; Autophagy related ubiquitin like modifier LC3 B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; LC
Gene Full Name:
Gene Infomation:
Antigen:Recombinant protein of human LC3A.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:Human
Reaction:Transfected Only.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:4C8-B7-B9
Dilution:WB 1:2000
Mole Mass:14/16kDa
Location:Cytoplasm ? cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle ? autophagosome membrane;Lipid-anchor
Concentration:6.9mg/mL
Sequence Similarity:
Gene Id:84557
SwissProt ID:Q9H492
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Background:Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.