Anti-LC3A Mouse mAb (8F5)
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Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:WB,IHC
Purify method:Affinity purified
Species:Human,Rat,Mouse
Gene Name:MAP1LC3A
Synonyms:ATG8E, MAP1LC3A, autophage-related protein LC3A
Gene Synonyms:
Gene Full Name:
Gene Infomation:
Antigen:Purified recombinant protein expressed in E.coli.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:
Reaction:The LC3A mouse monoclonal antibody can recognizeendogenous LC3A proteins.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:8F5-10H1-9A7
Dilution:WB 1:1,000-2,000 IHC 1:100-200 (Optimal dilutions should bedetermined by the end user)
Mole Mass:14,16kDa
Location:Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule
Concentration:
Sequence Similarity:
Gene Id:84557
SwissProt ID:Q9H492
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Background:Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600).
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.