Anti-LC3B Mouse mAb (8H6)

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:IHC

Purify method:Affinity purified

Species:Human

Gene Name:MAP1LC3B

Synonyms:ATG8F, MAP1 light chain 3 like protein 2, MAP1A/MAP1B LC3 B

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant protein expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:The LC3B mouse monoclonal antibody can recognizeendogenous LC3B proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:8H6-6D10-3A4

Dilution:IHC 1:100-200 (Optimal dilutions should bedetermined by the end user)

Mole Mass:14,16kDa

Location:Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule

Concentration:

Sequence Similarity:

Gene Id:81631

SwissProt ID:Q9GZQ8

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.