Anti-Lamin A/C Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Mouse IgG1

Host:Mouse

Application:WB,IF

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:LMNA

Synonyms:FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B

Gene Synonyms:70 kDa lamin ; Cardiomyopathy dilated 1A (autosomal dominant) ; CDCD1 ; CDDC ; CMD1A ; CMT2B1 ; EMD2 ; FPL ; FPLD ; FPLD2 ; HGPS ; IDC ; LAMIN A ; lamin A/C ; Lamin A/C like 1 ; Lamin ; LAMIN C ; Lamin-A/C ; LDP1 ; LFP ; LGMD1B ; Limb girdle muscular dystrophy 1B (autosomal dominant) ; LMN 1 ; LMN A ; LMN C ; LMN1 ; LMNA ; LMNA_HUMAN ; LMNC ; LMNL1 ; Prelamin A/C ; PRO1 ; Renal carcinoma antigen NY REN 32 ; Renal carcinoma antigen NY-REN-32 ; Renal carcinoma antigen NYREN32.

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human LMNA protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:Lamin A/C Mouse mAb detects endogenous levels of lamin A and lamin C proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:5D12-C6-E9

Dilution:WB 1:1000; IF 1:200

Mole Mass:

Location:Nucleus. Nucleus envelope. Nucleus lamina. Nucleus>nucleoplasm

Concentration:

Sequence Similarity:Belongs to the intermediate filament family.

Gene Id:

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the innernuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact withchromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role innuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal developmentof peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required forosteoblastogenesis and bone formation. Also prevents fat infiltration of muscle and bone marrow, helping tomaintain the volume and strength of skeletal muscle and bone Prelamin-A/C can accelerate smooth muscle cell senescence. It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and prematuresenescence.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.