Anti-MEK3/MEK6 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF,IP,FC

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; MAP2K6; MEK3; MAP kinase kinase 3; MAPKK3; MAPK/ERK kinase 3;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Activates acetate so that it can be used for lipid synthesis or for energy generation. Cytoplasmic acetyl-CoA synthetase (AceCS1) catalyzes the conversion of acetate and CoA to acetyl-CoA. Acetyl-CoA synthesized by AceCS1 is used for fatty acid and lipid biosynthesis. Studies suggest that this enzyme is regulated by sterol regulatory element-binding proteins. It was also shown that this enzyme is acetylated by protein acetyltransferases in cells. SIRT1 deacetylates this enzyme at Lys661 resulting in its activation and the increase of fatty acid synthesis.

Antigen:A synthesized peptide derived from human MEK3/MEK6

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:MEK3/MEK6 Antibody detects endogenous levels of MEK3/MEK6

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:1000 IHC 1:50~1:200 ICC/IF 1:100~1:500 IP 1:50 FC 1:50

Mole Mass:39,37kDa

Location:Cytoplasm;

Concentration:

Sequence Similarity:Belongs to the ATP-dependent AMP-binding enzyme family.

Gene Id:

SwissProt ID:P46734P52564

Unigene:

Nucleotide Accession:

Tissue specificity:MEK3/MEK6 Antibody detects endogenous levels of MEK3/MEK6

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.