Anti-MSH2 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Human

Gene Name:msh2

Synonyms:FCC1;COCA1;HNPCC;LCFS2;HNPCC1.

Gene Synonyms:BAT26;COCA 1;COCA1;DNA mismatch repair protein Msh2;FCC 1;FCC1;hMSH2;HNPCC 1;HNPCC;HNPCC1;LCFS2;MSH 2;Msh2;MSH2_HUMAN;MutS homolog 2;MutS homolog 2 colon cancer nonpolyposis type 1;MutS protein homolog 2.

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human MSH2 protein fragments expressed in E.coli

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of MSH2 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:4G5-E11-D9

Dilution:WB 1:1000

Mole Mass:100kDa

Location:Nucleus

Concentration:

Sequence Similarity:Belongs to the DNA mismatch repair MutS family.

Gene Id:4436

SwissProt ID:P43246

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:Component of the post-replicative DNA mismatch repair system (MMR).Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair.When bound,heterodimers bend the DNA helix and shields approximately 20 base pairs.MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA.MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding,MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer,which is thought to be responsible for directing the downstream MMR events, including strand discrimination,excision,and resynthesis.ATP binding and hydrolysis play a pivotal role in mismatch repair functions.The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange,resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone.This transition is crucial for mismatch repair.MutS alpha may also play a role in DNA homologous recombination repair.In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.