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Anti-Mannose Phosphate Isomerase Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Phosphoralated

Isotype:IgG1

Host:Mouse

Application:WB,ICC

Purify method:Affinity purified

Species:Human,Rat

Gene Name:mpi

Synonyms:PMI; PMI1; CDG1B

Gene Synonyms:PMI1;CDG1B;FLJ39201;Mannose 6 phosphate isomerase;Mannose-6-phosphate isomerase; MANNOSEPHOSPHATE ISOMERASE;MGC94106;MPI;MPI_HUMAN;Phosphohexomutase;phosphomannose isomerase 1;Phosphomannose isomerase;PMI;PMI1.

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human Mannose Phosphate Isomerase protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of Mannose Phosphate Isomerase and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

  • Western blot (1:100 to 1:500)
  • Immunofluorescence (1:50 to 1:400)
  • Immunohistochemistry (1:200 to 1:500)
  • Flow cytometry analysis (1:200 to 1:500)
  • Enzyme-linked Immunosorbent Assay (1:100-1:5000)
NoteUsers are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:4G9-B4-B8

Dilution:WB 1:1000,ICC 1:300

Mole Mass:54kDa

Location:Cytoplasm

Concentration:

Sequence Similarity:Belongs to the mannose-6-phosphate isomerase type 1 family.

Gene Id:4351

SwissProt ID:P34949

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol

Background:Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.