Anti-NEDD8 Rabbit pAb
- 100ug (NP21395) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,IHC,ICC/IF,IP
Purify method:Affinity Chromatography
Species:Human,Mouse,Rat
Gene Name:
Synonyms:NEDD8; Nedd-8;
Gene Synonyms:
Gene Full Name:
Gene Infomation:Caspases are a family of cytosolic aspartate specific cysteine proteases. Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaved Caspase 10 leads to the processing of Caspase 3 and Caspase 7, initiating a caspase cascade and subsequent apoptosis.
Antigen:A synthesized peptide derived from human NEDD8
Antigen Synonyms:
Clonality:Polyclonal antibody
Source:
Reaction:NEDD8 Antibody detects endogenous levels of total NEDD8
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:20
Mole Mass:10,90kDa
Location:
Concentration:
Sequence Similarity:Contains 2 DED (death effector) domains.
Gene Id:
SwissProt ID:Q15843
Unigene:
Nucleotide Accession:
Tissue specificity:NEDD8 Antibody detects endogenous levels of total NEDD8
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Background:Neural precursor cell-expressed developmentally downregulated protein 8 (NEDD8), also known as Rub1 (related to ubiquitin 1) in plants and yeast, is a member of the ubiquitin-like protein family. The covalent attachment of NEDD8 to target proteins, termed neddylation, is a reversible, multi-step process analogous to ubiquitination.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.