Anti-NFκB-p105/p50 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Rabbit

Application:WB,IHC,ELISA

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:NFKB1

Synonyms:NFKB1; Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Gene Synonyms:p50; KBF1; p105; EBP-1; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

Gene Full Name:

Gene Infomation:This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. [provided by RefSeq, Feb 2016]

Antigen:Synthesized peptide derived from human NFκB-p105/p50 around the non-phosphorylation site of S337.

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB::1/500-1/2000IHC::1/100-1/300

Mole Mass:

Location:Cytoplasm;

Concentration:

Sequence Similarity:Contains 1 RHD (Rel-like) domain.

Gene Id:4790

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:NFκB-p105/p50 Polyclonal Antibody detects endogenous levels of NFκB-p105/p50 protein.

Storage:

Buffer condition:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.