Anti-NFKB(p100) (C-term S866/870) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:Rabbit Ig

Host:Rabbit

Application:WB

Purify method:Affinity purified

Species:Human

Gene Name:

Synonyms:Nuclear factor NF-kappa-B p100 subunit, DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10 protein, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, Oncogene Lyt-10, Lyt10, Nuclear factor NF-kappa-B p52 subunit, NF

Gene Synonyms:

Gene Full Name:

Gene Infomation:NF-kappa-B has been detected in numerous cell types thatexpress cytokines, chemokines, growth factors, cell adhesionmolecules, and some acute phase proteins in health and in variousdisease states. NF-kappa-B is activated by a wide variety ofstimuli, such as cytokines, oxidant-free radicals, inhaledparticles, ultraviolet irradiation, and bacterial or viralproducts. Inappropriate activation of NF-kappa-B has been linked toinflammatory events associated with autoimmune arthritis, asthma,septic shock, lung fibrosis, glomerulonephritis, atherosclerosis,and AIDS. In contrast, complete and persistent inhibition ofNF-kappa-B has been linked directly to apoptosis, inappropriateimmune cell development, and delayed cell growth. NFKB1 (MIM164011) and NFKB2 encode p105 and p100 proteins that are processedto produce the active p50 and p52 NF-kappa-B subunits,respectively. However, the p100 and p105 proteins serve regulatoryfunctions and should not be considered exclusively as precursorforms. The most abundant activated form of NF-kappa-B is aheterodimer of the p50 or p52 subunit bound to the RELA subunit(MIM 164014). Other NF-kappa-B complexes, consisting of hetero- andhomodimers of p50, p52, RELA, REL (MIM 164910), and RELB (MIM604758), have also been detected. NF-kappa-B complexes areinhibited by I-kappa-B proteins, NFKBIA (MIM 164008) or NFKBIB (MIM604495), which inactivate NF-kappa-B by trapping it in thecytoplasm. Phosphorylation of serine residues on the I-kappa-Bproteins by the kinases IKBKA (CHUK; MIM 600664) or IKBKB (MIM603258) marks them for destruction via the ubiquitination pathway,thereby allowing activation of the NF-kappa-B complex. Theactivated NF-kappa-B complex translocates into the nucleus andbinds DNA at kappa-B-binding motifs, such as 5-prime GGGRNNYYCC3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R isan A or G purine; and Y is a C or T pyrimidine). For reviews, seeChen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed8717528].

Antigen:This NFKB(p100) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 848-874 amino acids from the C-terminal region of human NFKB(p100).

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:HUMAN

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB::1:1000

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Gene Id:

SwissProt ID:

Unigene:

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Tissue specificity:

Storage:

Buffer condition:Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Background:

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Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.