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Anti-NFkB p100 / p52 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,ICC/IF,IP

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:CVID10; DNA binding factor KBF2; H2TF1; Lyt10; NFKB2; Nuclear factor NF kappa B p100 subunit; Nuclear factor NF kappa B p52 subunit; Oncogene Lyt 10; p105; p49/p100;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin

Antigen:A synthesized peptide derived from human NFkB p100 / p52

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:NFkB p100 / p52 Antibody detects endogenous levels of total NFkB p100 / p52

Form:Liquid

Tested Applications:

  • Western blot (1:100 to 1:500)
  • Immunofluorescence (1:50 to 1:400)
  • Immunohistochemistry (1:200 to 1:500)
  • Flow cytometry analysis (1:200 to 1:500)
  • Enzyme-linked Immunosorbent Assay (1:100-1:5000)
NoteUsers are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:1000~1:5000 ICC/IF 1:50~1:200 IP 1:50

Mole Mass:120,52kDa

Location:Cytoplasm;

Concentration:

Sequence Similarity:Contains 1 RHD (Rel-like) domain.

Gene Id:

SwissProt ID:Q00653

Unigene:

Nucleotide Accession:

Tissue specificity:NFkB p100 / p52 Antibody detects endogenous levels of total NFkB p100 / p52

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.