Anti-NFkB-p65 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Rabbit

Application:WB,IHC

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:rela

Synonyms:Avian reticuloendotheliosis viral (v rel) oncogene homolog A;MGC131774;NF kappa B p65delta3;NFKB 3;NFKB3;Nuclear Factor NF Kappa B p65 Subunit;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B cells 3;Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3;OTTHUMP00000233473;OTTHUMP00000233474;OTTHUMP00000233475;OTTHUMP00000233476;OTTHUMP00000233900;p65;p65 NF kappaB;p65 NFkB;RELA;TF65_HUMAN;Transcription Factor p65;v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65));V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A;v rel reticuloendotheliosis viral oncogene homolog A (avian);V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65;v-rel reticuloendotheliosis viral oncogene homolog A.

Gene Synonyms:p65; NFKB3

Gene Full Name:

Gene Infomation:NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Antigen:The antiserum was produced against synthesized non-phosphopeptide derived from human NFkB-p65 around the phosphorylation site of serine 536.

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:Human

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:

Mole Mass:69

Location:Nucleus. Cytoplasm. Note: Colocalized with DDX1 in the nucleus upon TNF-alpha induction By similarity. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalizes with GFI1 in the nucleus after LPS stimu

Concentration:

Sequence Similarity:Contains 1 RHD (Rel-like) domain.

Gene Id:5970

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:This antibody detects endogenous levels of NF-Kb p65 and does not cross-react with related proteins.

Storage:

Buffer condition:10mM PBS with 0.02% sodium azide, 50% glycerol.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.