Anti-NGFR Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Mouse IgG1

Host:Mouse

Application:WB,ICC,FCM

Purify method:Ascitic fluid

Species:Human

Gene Name:NGFR

Synonyms:CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR; NGFR

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant fragment of human NGFR expressed in E. Coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:2F1C2

Dilution:WB: 1/500 - 1/2000; IHC: N/A; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; Elisa: 1/10000

Mole Mass:45kDa

Location:

Concentration:

Sequence Similarity:

Gene Id:4804

SwissProt ID:P08138

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Ascitic fluid containing 0.03% sodium azide.

Background:Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGFR p75 plays a central role in the regulation of cell number by apoptosis in the developing CNS. During early development, activation of NGFR p75 by NGF induces apoptotic cell death in some neuronal cells, probably through activation of the sphingomyelinase/ceramide pathway, the ICE like proteases and the JNK pathway. In rat Schwann cells, NGF binding to NGFR p75 activates NF kappaB, possibly to modulate Schwann cell migration during nerve regeneration. CD271 has recently been described as being expressed in mesenchymal stem cells (bone marrow stromal cells).

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.