Anti-Nestin (C-term) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:Rabbit Ig

Host:Rabbit

Application:IHC-P,IF

Purify method:Affinity purified

Species:Human,Mouse

Gene Name:nestin

Synonyms:Nestin, NES

Gene Synonyms:

Gene Full Name:

Gene Infomation:Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments.Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.

Antigen:This Nestin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1560~1590 amino acids from the C-terminal region of human nestin.

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:HUMAN

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:RB2857

Dilution:ICC::1:10~50IHC::1:50~100

Mole Mass:

Location:

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:

Buffer condition:Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.