Anti-Osteopontin Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,ICC/IF,FC

Purify method:Affinity Chromatography

Species:Human,Mouse

Gene Name:Osteopontin

Synonyms:BNSP; Bone sialoprotein 1; bone sialoprotein I; BSPI; early T-lymphocyte activation 1; ETA-1; Nephropontin; OPN; Osteopontin; OSTP; Secreted phosphoprotein 1;

Gene Synonyms:OPN; BNSP; BSPI; ETA-1.

Gene Full Name:

Gene Infomation:The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.

Antigen:A synthesized peptide derived from human Osteopontin

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Osteopontin Antibody detects endogenous levels of total Osteopontin

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:2000 ICC/IF 1:50~1:200 FC 1:30

Mole Mass:35kDa

Location:Secreted.

Concentration:

Sequence Similarity:Belongs to the osteopontin family.

Gene Id:6696

SwissProt ID:P10451

Unigene:

Nucleotide Accession:

Tissue specificity:Osteopontin Antibody detects endogenous levels of total Osteopontin.

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:osteopontin Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. Ligand for integrin alpha-V/beta-3. 4 isoforms of the human protein are produced by alternative splicing.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.