Anti-PCNA (C-term) Mouse mAb
- 100ug (NP31312) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:
Isotype:IgG1
Host:Mouse
Application:IHC-P,WB
Purify method:Affinity purified
Species:Human,Mouse,Rat
Gene Name:pcna
Synonyms:Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Gene Synonyms:
Gene Full Name:
Gene Infomation:This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Antigen:This PCNA antibody is generated from miceimmunized with a KLH conjugated synthetic peptide between 236-261 amino acids from the C-terminal region of human PCNA.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:Human
Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:1157CT1.1.3
Dilution:WB::1:2000IHC::1:25
Mole Mass:
Location:
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Sequence Similarity:
Gene Id:
SwissProt ID:
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:
Buffer condition:Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Background:
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.